حسین عزیزی

بیوگرافی :

مدارک علمی :

زمینه‌های تدریس :

• زیست شناسی سلولی و مولکولی
• مبانی سلول های بنیادی
• کشت سلول
• کشت بافت جانوری

Journal Papers:

1. Reza E, Azizi H. Correlation Between Alkaline Phosphatase Expression and Sox2, Oct4, and Nanog Genes in Spermatogonial and ES-Like Cells. Regenerative Engineering and Translational Medicine. 2023 Aug 9:1-1.
2. Reza E, Azizi H, Skutella T. Sox2 Localization During Spermatogenesis and Its Association with other Spermatogenesis Markers Using Protein-Protein Network Analysis. Journal of Reproduction & Infertility. 2023 Aug 2;24(3):171-80.
3. Darzi MB, Nemati F, Azizi H, Jouybari AD. Immunohistochemistry and immunocytochemistry analysis of PLZF and VASA in mice testis during spermatogenesis. Zygote. 2023 Jun;31(3):273-80.
4. Mehdinezhad Roshan M, Azizi H. Advanced isolation, expansion and characterization research study on pig testicular cells during differentiation and proliferation. Animal Biotechnology. 2023 May 2:1-8.
5. Hashemi Karoii D, Azizi H. OCT4 protein and gene expression analysis in the differentiation of spermatogonia stem cells into neurons by immunohistochemistry, immunocytochemistry, and bioinformatics analysis. Stem Cell Reviews and Reports. 2023 Apr 29:1-7.
6. Sojoudi K, Azizi H, Skutella T. A Fundamental Research in In Vitro Spermatogonial Stem Cell Culturing: What Are Clump Cells?. Cellular Reprogramming. 2023 Apr 1;25(2):65-72.
7. Sojoudi K, Azizi H, Skutella T. A review of the potential of induced pluripotent stem cell-derived exosome as a novel treatment for male infertility. Biotechnology and Genetic Engineering Reviews. 2023 Mar 25:1-26.
8. Reza E, Azizi H. Effects of Alkaline Phosphatase, GATA, and BMP Expression on Three Populations of Cells: Spermatogonial Stem Cells, Embryonic Cells, and Embryonic Stem-Like Cells in Mice. Journal of Gorgan University of Medical Sciences. 2023 Mar 10;25(1):29-37.
9. Reza E, Azizi H, Ahmadi AA. Evaluation and Comparison of the Expression Levels of the ZBTB16 (Plzf) and ZFP Genes and Alkaline Phosphatase in Three Cell Populations: Mouse Spermatogonial Stem Cells, Embryonic Stem-Like Cells (Es-Like), And Embryonic Stem Cells. Journal of Ilam University of Medical Sciences: Volume. 2023;31(1).
10. Masoudi M, Azizi H, Sojoudi K, Yazdani M, Gholami D. Comparison of POU5F1 gene expression and protein localization in two differentiated and undifferentiated spermatogonial stem cells. Biologia Futura. 2022 Dec;73(4):503-12.
11. Hashemi Karoii D, Azizi H, Skutella T. Microarray and in silico analysis of DNA repair genes between human testis of patients with nonobstructive azoospermia and normal cells. Cell Biochemistry and Function. 2022 Dec;40(8):865-79.
12. Azizi H, Hashemi Karoii D, Skutella T. Whole exome sequencing and in silico analysis of human Sertoli in patients with non-obstructive azoospermia. International Journal of Molecular Sciences. 2022 Oct 20;23(20):12570.
13. Amirian M, Azizi H, Hashemi Karoii D, Skutella T. VASA protein and gene expression analysis of human non-obstructive azoospermia and normal by immunohistochemistry, immunocytochemistry, and bioinformatics analysis. Scientific Reports. 2022 Oct 14;12(1):17259.
14. Hashemi Karoii, D., & Azizi, H. (2022). A review of protein-protein interaction and signaling pathway of Vimentin in cell regulation, morphology, and cell differentiation in normal cells. Journal of Receptors and Signal Transduction, 1-9.
15. Niazi Tabar, A., Azizi, H., Hashemi Karoii, D., & Skutella, T. (2022). Testicular Localization and Potential Function of Vimentin Positive Cells during Spermatogonial Differentiation Stages. Animals, 12(3), 268.
16. Niknejad, P., Azizi, H., & Sojoudi, K. (2021). POU5F1 Protein and Gene Expression Analysis in Neonate and Adult Mouse Testicular Germ Cells by Immunohistochemistry and Immunocytochemistry. Cellular Reprogramming, 23(6), 349-358.
17. Azizi, H., Niazi Tabar, A., & Skutella, T. (2021). Successful transplantation of spermatogonial stem cells into the seminiferous tubules of busulfan-treated mice. Reproductive health, 18(1), 1-9.
18. Azizi, H., NiaziTabar, A., Mohammadi, A., & Skutella, T. (2021). Characterization of DDX4 gene expression in human cases with non-obstructive azoospermia and in sterile and fertile mice. Journal of Reproduction & Infertility, 22(2), 85.
19. Azizi, H., Niazi Tabar, A., & Mohammadi, A. (2020). Experimental investigation of Ki67, POU5F1, and ZBTB16 expression in the pig and mouse testicular cells using immunocytochemistry and RT-PCR. Journal of Ilam University of Medical Sciences, 28(5), 1-10.
20. Azizi, H., Tabar, A. N., Skutella, T., & Govahi, M. (2020). In vitro and in vivo determinations of the anti-GDNF family receptor alpha 1 antibody in mice by immunochemistry and RT-PCR. International Journal of Fertility & Sterility, 14(3), 228.
21. Azizi H, Ranjbar M, Rahaiee S, Govahi M, Skutella T. Investigation of VASA gene and protein expression in neonate and adult testicular germ cells in mice in vivo and in vitro. Cell Journal (Yakhteh) "Volume 22, Number 2, Jul-Sep (Summer) 2020, Serial Number: 86.
22. Azizi H, Koruji M, Skutella T. PLZF gene expression in pluripotent stem cells and testicular germ cells. Volume 22, Number 1, Apr-Jun (Spring) 2020, Serial Number: 85.
23. Azizi H, Asgari B, Skutella T. "Pluripotency potential of testis-derived embryonic stem cell like cells derived from mouse testis". Cell Journal (Yakhteh). Volume 21, Number 3, Autumn 2019, Serial Number: 83.
24. Azizi H, Conrad S, Skutella T. Differential Proliferation Effects after Short-term Cultivation of Mouse Spermatogonial Stem Cells on Different Feeder Layers. Cell Journal (Yakhteh) Volume 21, Number 2, Jul-Sep (Summer) 2019, Serial Number: 82.
25. Conrad S, Azizi H, Skutella T, Single-Cell Expression Profiling and Proteomics of Primordial Germ Cells, Spermatogonial Stem Cells, Adult Germ Stem Cells, and Oocytes. Adv Exp Med Biol. 2018 Jan 4. doi: 10.1007/5584_2017_117.
26. Azizi H, Skutella T, Shahverdi A, Generation of Mouse Spermatogonial Stem-Cell-Colonies in A Non-Adherent Culture. Cell Journal. 2017 Jul-Sep;19(2):238-249. doi: 10.22074/cellj.2016.4184. Epub 2017 Feb 22.
27. Bojnordi M, Azizi H, Skutella T, Movahedin M, Pourabdolhossein F, Shojaei A, Hamidabadi H. Differentiation of Spermatogonia Stem Cells into Functional Mature Neurons Characterized with Differential Gene Expression. Mol Neurobiol. 2016 Sep 19.
28. Azizi H, Skutella T, Shahverdi A. A Generation of Mouse Spermatogonial Stem-Cell-Colonies in Non-adherent Culture. Cell Journal (Yakhteh) Volume 19, Number 2, Summer 2017, Serial Number: 74.
29. Azizi H, Conrad S, Hinz U, Asgari B, Nanus D, Peterziel H, Moghaddam A, Baharvand H, Skutella T. Derivation of Pluripotent Cells from Mouse SSCs Seems to Be Age Dependent. Stem Cells International, Volume 2016 (2016), Article ID 8216312, 13 pages.

Research Projects :

1. جداسازی و کشت سلول های بنیادی اسپرم ساز و بررسی تمایز آنها به اسپرم بالغ در کمک به درمان ناباروری
2. بررسی و مقایسه الگوی بیان ژن های شاخص سلول های شبه سلول های بنیادی جنینی (ES-like) با سلول های بنیادی اسپرم ساز و تأثیر عملکردی آن بر ناباروری با هدف توسعه درمانی جدید
3. بررسی کشت و پتانسیل پرتوانی سلولهای بنیادی اسپرم ساز موشی
4. تولید خودبه خودی سلول های بنیادی پرتوان از بیضه موش نوزاد
5. نگهداری رده سلول های بنیادی اسپرم ساز
6. بررسی کشت و پتانسیل پرتوانی سلول های بنیادی اسپرم ساز موشی
7. شناسایی ژن های جنسی مهم در سلول های بنیادی اسپرم ساز